Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry.

Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 muL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.

Methods Mol Biol. 2017;1619:203-212. doi: 10.1007/978-1-4939-7057-5_16.
Authors:
S. Sales, Oskar Knittelfelder, A. Shevchenko
Date Published:
Journal:
Methods Mol Biol